What units are used to measure absorbance on a spectrophotometer

Asked by admin @ 28/10/2021 in Chemistry viewed by 366 People

A student measures the absorbance of a sample of red dye #3 using a spectrophotometer. If the absorbance is measured as 0.468, what is the concentration of red dye #3 in the sample?

Enter your answer in units of micromolar to three significant figures.
n the lab spectrophotometer, d = 1.00 cm. For Red dye #3, ε = 7.96 x 104 M-1 cm-1.

Answered by admin @ 28/10/2021

Red dye #3 with a concentration of 5.88 μM has an absorbance of 0.468 in the lab spectrophotometer.

A student measures the absorbance of a sample of red dye #3 using a spectrophotometer and the value is A = 0.468. We can calculate the concentration of the ample using the Beer-Lambert law, which relates the attenuation of light to the properties of the material through which the light is travelling, through the following equation.

$A = \epsilon \times d \times c$

where,

ε: molar absorptivity of the species (7.96 × 10⁴ M⁻¹ cm⁻¹)
d: optical path length (1.00 cm)
c: concentration

$c = \frac{A}{\epsilon \times d } = \frac{0.468}{7.96 \times 10^{4} M^{-1}cm^{-1} \times 1.00cm } \times \frac{10^{6} \mu M }{1M} = 5.88 \mu M$

Red dye #3 with a concentration of 5.88 μM has an absorbance of 0.468 in the lab spectrophotometer.

Learn more: brainly.com/question/20956841

What units are used to measure absorbance on a spectrophotometer

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