Can a live specimen be used in a light microscope

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Compare and contrast light microscopes, scanning electron microscopes, and transmission electron microscopes. Sort each statement into the correct box. Some statements may apply to more than one type of microscope. Some statements may not be used. Use a beam of electrons to produce magnified images added to Scanning Electron MicroscopesUse a beam of electrons to produce magnified images Can be used to examine living cells and tissues Can be used to examine DNA Light microscopes Use a beam of light to produce magnified imagesCan be used to examine cells Scanning Electron Microscopes Can be used to examine cellsCan be used to examine DNAUse a beam of electrons to produce magnified images Transmission Electron Microscopes Can be used to examine cells

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Answer:

Light microscope:

  • use a beam of light to produce magnified images
  • can be used to examine living cells and tissues

Scanning electron microscope:

  • use a beam of electrons to produce magnified images
  • can be used to examine DNA
  • can be used to examine cells

Transmission electron microscope:

  • use a beam of electrons to produce magnified images
  • can be used to examine DNA
  • can be used to examine cells

Explanation:

Light microscope: is a commonly used microscope also known as compound microscope. Magnifies images from 40X upto 1000X. It uses ray of visible light to produce a magnified image. The light microscope can be used to view specimen of both living and dead cells or tissues. However, it doesn't give a detailed view of a specimen like electron microscope.

Scanning electron microscope: It uses electron beam as an illuminating source. It has a much higher resolving power than light microscope because it uses electrons instead of light. It magnifies object upto 500000 times the actual size. Internal structures can also be viewed. However, only dead specimen can be used because the beam of electrons can kill the cells. They are of two types:

  1. Scanning electron microscope(SEM): an electron beam passes over the specimen's surface and displaces electrons which are then focused on  a screen to form an image. Images appear in 3-D
  2. Transmission electron microscope: electromagnets magnify the image by passing beam of electrons through a thin specimen. Images appear in 2-D


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